[Solved]: 5′ Nucleotidase is an enzyme that hydrolyzes 5’…

5′ Nucleotidase is an enzyme that hydrolyzes 5′ nucleotides to 5′ nucleosides at an optimal pH of 7.5. It serves as an important marker of cholestasis, showing moderate elevation in hepatitis and significant elevation in biliary obstruction. Unlike alkaline phosphatase (ALP), which can be elevated in both liver and bone disorders, 5′ nucleotidase levels are not affected by bone disease or osteoblastic activity. This specificity makes it a valuable diagnostic tool to differentiate between liver-related and bone-related causes of elevated ALP.